| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cao, Cecilia Y (12)
|
ADDELM |
39.72 |
40.20 |
0.48 |
| 2 |
Jerabek, Ulka G (12)
|
ADDELM |
40.20 |
41.08 |
0.88 |
| 3 |
Czajkowski, Izabella (12)
|
ADCPP |
43.27 |
43.80 |
0.53 |
| 4 |
Bonesteel, Carsyn E (12)
|
ADUN |
43.23 |
44.21 |
0.98 |
| 5 |
Sorbello, Courtney H (11)
|
ADDELM |
42.75 |
45.85 |
3.10 |
| 6 |
Skuza, Olivia (11)
|
ADDELM |
50.53 |
46.46 |
-4.07 |
| 7 |
Curtis, Amanda E (12)
|
ADCPP |
45.51 |
47.61 |
2.10 |
| 8 |
Tisenchek, Isabella S (12)
|
ADCPP |
50.89 |
47.71 |
-3.18 |
| 9 |
Baldwin, Elizabeth A (11)
|
ADDELM |
46.92 |
47.94 |
1.02 |
| 10 |
Clarke, Charlotte E (11)
|
ADDELM |
47.04 |
48.91 |
1.87 |
| 11 |
Silaika, Sophia G (12)
|
ADCPP |
49.00 |
50.19 |
1.19 |
| 12 |
Reed, Samantha M (12)
|
ADCPP |
54.06 |
51.60 |
-2.46 |
| 13 |
Gayle, Isabella C (11)
|
ADUN |
52.49 |
52.07 |
-0.42 |
|
Cioccke, Isabella K (12)
|
ADCPP |
1:03.99 |
DQ |
- |
|
Narisetty, Tejasree (11)
|
ADCPP |
1:17.96 |
DQ |
- |
|
Choma, Isabel (12)
|
ADUN |
54.45 |
NS |
- |